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Journal: Journal of Thoracic Disease
Article Title: Glycyrrhizic acid attenuates sepsis-induced acute lung injury by regulating macrophage M2 polarization via PRKCD
doi: 10.21037/jtd-2026-1327
Figure Lengend Snippet: GA promotes M2 polarization via PRKCD to alleviate SI-ALI. (A) Immunohistochemistry analysis of PRKCD expression. (B) Hematoxylin and eosin staining of lung tissues. (C) Assessment of pulmonary edema by W/D ratio. (D) Immunofluorescence analysis of iNOS and Arg1 expression in lung tissues. (E) ELISA analysis of IL-1β and TNF-α levels in BALF. Data are presented as mean ± SD (n=6 mice per group). For multiple-group comparisons, one-way ANOVA followed by Tukey’s post hoc test was applied. *, P<0.05; ***, P<0.001; ****, P<0.0001. ANOVA, analysis of variance; BALF, bronchoalveolar lavage fluid; DAPI, 4',6-diamidino-2-phenylindole; ELISA, enzyme-linked immunosorbent assay; GA, glycyrrhizic acid; IL-1β, interleukin-1 beta; NC, negative control; PRKCD, protein kinase C delta; SD, standard deviation; SI-ALI, sepsis-induced acute lung injury; TNF-α, tumor necrosis factor-alpha; W/D, wet-to-dry weight.
Article Snippet: Samples were incubated overnight at 4 °C with primary antibodies:
Techniques: Immunohistochemistry, Analysis, Expressing, H&E Stain, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Negative Control, Standard Deviation